Restriction Mapping

Restriction Mapping Dry Lab Activity 1.Below is a obstacle use for the plasmid pGEN101 (total length = 20 kb). absorb this chromosome mapping as a guide, give the number of regionuriency fragments along with their associated lengths that would result from subscribeing pGEN101 with the breastwork enzymes EcoRI, BamHI, and a combination of EcoRI + BamHI. hold out(a) Performed:Sizes of Fragments Obtained: EcoRI BamHI.. EcoRI + BamHI... 2.Two freshmen college bookmans, interested in becoming gene jocks, performed the pursuance fasten of parturiency digests on a newly detached plasmid, pBLA230. The chemical reaction they carries out, along with the fragment obtained in single and reduplicate digest reactions were: Enzyme(s)Fragment Lengths Obtained: HpaI26 kb HindIII13 kb, 6 kb, 4 kb, 3 kb HpaI + HindIII7 kb, 6 kb (2), 4 kb, 3 kb Using this information, come the restriction map of pBLA230. 3.Plasmid pBR607 is a 2.6 kb plasmid containing ampicillin and Tetracycline resistance markers, an simple eye of replication, and unique restriction sites for the restriction enzymes EcoRI, BamHI, and PstI.

Given the restriction map for pBR607 for the enzymes EcoRI, BamHI, and PstI, show on the agarose gel imprint below where the rough positions of the restriction fragments generated from the given restriction digests would be located after carrying out electrophoresis. 4.As part of an undergraduate project, a student was attempting to construct a restriction map for the plasmid pUC23 victimization the restriction enzymes EcoRI and BamHI. After carrying out both single and twice enzyme digest reacti ons and electrophoresing to from each one ! angiotensin-converting enzyme reaction mix through an aragose gel, the picture below is obtained, showing the number of DNA fragments produced in each reaction, along with the sizes of each fragment. From this information, construct a restriction map of the pUC23 for enzymes EcoRI and BamHI....If you want to ascertain a full essay, order it on our website: OrderEssay.net

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