Crime Lab

Alex Powell
Chemistry Lab
4/5/2012
Crime Laboratory
I. In this testing ground you are finding the concentration of your unknown which isnt a real blood serum. You are using a unmistakable spectroscopy which is the measurement of the amount of light absorbed by a solution. My hypothesis is that this method is valid and reliable.
II. Some materials employ in this lab were spectrophotometer cuvettes, test tube rack, pipet, veriner and a spectrophotometer.
third. world-class thing you do is you place seven spectrophotometer cuvettes in a small test tube rack. Make sure you represent along the rigid side because fingerprints on the clear sides bottom mess up the readings. You then pipet the amounts of material listed in Table 1 of your lab manual, then shake the minglees thoroughly. numeral on you spectrophotometer and set the wavelength dial 540 nm. Allow the instrument to tippy up for ten minutes. Then prepare the unknown warning by pipetting 1 ml of the unknown solution into the cuvette and mix with 1 ml of trim back ferric nitrate. Then after(prenominal) that you calculate the concentration for each of the solutions.

Cuvette #| 25 mg/dl Standard| H2O ml| Dilute Iron III Nitrate (ml)| .039 M HNO3 (ml)| Concentration|
1| 0| 1| 0| 1| 0|
2| 0.1| 0.9| 1| 0| 0.09|
3| 0.3| 0.7| 1| 0| 0.287|
4| 0.5| 0.5| 1| 0| 0.



407|
5| 0.7| 0.3| 1| 0| 0.706|
6| 1| 0| 1| 0| 1.316|

When then use the calibration skid to determine the concentration of the unknown and we get .747.
IV. As you basin see from our data as the amount of H2O decreases and the 25mg/dl tired increases as does our concentration for the salicylic acids. We keep the amount of dilute iron III nitrate the same because its our freelancer variable. With the unknowns concentration we know it falls between .7mbg/dl of standard and only .3 ml of water. My guess is that its just high the .7 because the concentration is just a little bit higher(prenominal) so maybe .71 or .72. The dependent variable in this lab is the...If you want to get a full essay, do it on our website: Orderessay



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